Journal: Gene therapy

Article Title: Imaging gene delivery in a mouse model of congenital neuronal ceroid lipofuscinosis

doi: 10.1038/gt.2011.118

Figure Lengend Snippet: NIRF imaging of CathD activity ex vivo . Neonatal pups were injected with AAV2/rh8-mCathD vector both i.c.v. (2 × 10 10 g.c into each ventricle in 2 μl 0.06% Trypan blue) and i.p. (1 × 10 10 g.c in 100 μl) on P1. Similar groups of mice were injected with PBS (n = 6/group). On P24 (2–3 days prior to mortality in knock-out animals), mice were injected i.p. with NIRF probe (0.1 nmoles/g body weight) and sacrificed 24 h later with PBS perfusion. Liver, brain, heart, spleen and kidneys were removed, rinsed with PBS, and imaged at 5 different regions of interests using a custom-built camera system followed by image analysis using Kodak Digital Science 1D software. B).( A ) representative organs from a single mouse in each group is shown.( B ) Signal intensity is expressed as the average relative light units (RLU) ± S.D. (n = 6). Significant increases in AAV-mCathD treated animals were seen for liver (*p<0.0001) and brain (**p<0.00001) as calculated by student t-test.

Article Snippet: Organs were imaged using a custom-built camera system followed by image analysis using Kodak Digital Science 1D software ( ).

Techniques: Imaging, Activity Assay, Ex Vivo, Injection, Plasmid Preparation, Knock-Out, Software